Mar. 30th, 2010

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.....yeah, I admit that sent me for a discography.

awakening_dreams: short skirt, long legs (Default)
Maybe the fact I was just re-bit will be a blessing and not a curse.

New test for Lyme disease
Updated: Monday, 29 Mar 2010, 6:00 PM EDT
Published : Monday, 29 Mar 2010, 5:12 PM EDT

Jocelyn Maminta
Milford, Conn. (WTNH) - A new test will help diagnose Lyme disease earlier, leading to more effective treatment.

As warm weather approaches so does the season of ticks and Lyme disease. Infected deer ticks are the culprits.

"This is a definitive molecular diagnosis of Lyme disease," explained Dr. Sin Hang Lee, Milford Hospital.

It took a year and a half in the research lab at Milford Hospital for Dr. Lee to develop a definitive way to diagnose Lyme disease using the DNA of the Lyme bacteria.

"This is the first using DNA sequencing to confirm diagnosis of Lyme spirochete DNA," Dr. Lee said.

But the window of opportunity is short. Key to this test is when the blood is drawn. Dr. Lee says it should be done on the onset of symptoms - clear evidence the bacteria is in a patient's blood.

"Every patient is different because from the time of the tick bite to symptoms, it may take three days, it may take 30 days," he said.

The sample is amplified twice to ensure a DNA match. Dr. Lee points out if there's a positive result it leads to early treatment.

More on the test has been publishes in the latest American Journal of Clinical Pathology.

In addition, I bit the bullet.

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My intended major is Audio Art and Acoustics. Fancy.
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For once I'm not paying attention to my post count, because this is important to repost.
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Dude.I really hate it when I am not told whether something's a joke on me or a real thing.

La nozionismo, li confonde me. Not the right time to do so!
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Connecticut Pathologist Debuts Lyme Disease Test Based on Nested PCR, DNA Sequencing
March 30, 2010

By Kirell Lakhman

A scientist in Connecticut has launched a test that uses nested PCR and DNA sequencing to detect the presence of Borrelia burgdorferi, the bacterium that causes Lyme disease, according to a statement today.

"Most insurance companies have already agreed to cover the cost for their members," the statement said, without elaborating.

The test, called LoTemp, makes its debut ahead of the spring and summer tick season in the eastern US.

Other PCR-based tests for the indication exist, "[b]ut this is the first … one using nested PCR for detection and DNA sequencing to validate the molecular diagnosis in clinical laboratory medicine."

The assay, developed by Sin Hang Lee, a pathologist at Milford Hospital in Milford, Conn., is can be used before patients undergo traditional serology testing for the bug. More than 30,000 people in the US are suspected to be infected with the spirochete B. burgdorferi each year.

The test uses nested PCR to detect genomic DNA of B. burgdorferi in blood, and uses Sanger-based DNA sequencing and diagnostic signature sequences found in GenBank to validate the result, according to the statement.

Up to 75 percent of patients with "acute-phase Lyme disease are negative for the characteristic antibodies, but in fact the percentage is higher," the statement said. However, a negative result does not rule out the presence of the bacteria because spirochetemia, or the presence of spirochetes in the blood, "is transient and its time points in Lyme [disease] vary from patient to patient."

A paper co-authored about the test, which appears in the current of the American Journal of Clinical Pathology, said the test "may be a valuable supplement to the current serologic tests for Lyme disease."

"It is the marriage of [nested PCR and DNA sequencing] that minimizes false-negatives to the lowest possible and eliminates false-positives known to be associated with other Lyme disease DNA tests," it said.

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